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How to Calculate Bacterial Concentration in Original SampleYou will be able to use the counted number to back-calculate (extrapolate) the amount ofbacterial cells in the original sample. Take the number of counted colonies and multiply bythe dilution factor.For example, if 67 colonies are counted on a plate that received 1.0 mL of the 1:1,000,000dilution, the calculation would be:(67 CFUs/mL x 1,000,000 dilution factor) = 67 x 10^6 bacteria per mL of undiluted cultureWhen calculating original sa
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