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The scope of the presented studies is somewhat limited, as they primarily focus on evaluating the phenotypic changes in cardiac innervation following the loss of various Slit or Robo genes.
This important work presents a self-supervised method for the segmentation of 3D cells in fluorescent microscopy images, conveniently packaged as a Napari plugin and tested on an annotated dataset.
Moreover, it was able to distinguish between polystyrene and polymethyl methacrylate beads within sub-diffraction aggregates, which was impossible with standard fluorescence microscopy.