iv. Be sure all connections are firm! b. Attach rubber tubing to the vacuum line. c. Vigorously shake the water bottle containing the 1 mL sample (diluted to 100 mL in water) to disperse the bacteria.

Three 100-mg samples of the powder ... to bring the final volume to 400 mL. Mix well. Transfer the suspension to an amber polyethylene terephthalate bottle with a child-resistant cap.