The supernatant plasma was decanted through an 18-gauge needle bent to an angle of 90° into 50-ml plastic centrifuge tubes. Two volumes of 0.87 per cent ammonium chloride were added to the plasma.

This was done by placing them in 50ml centrifuge tubes with tiny breathing holes, restricting their movement for 4 hours each day over a period of three weeks. The researchers used Open-field ...

After 72 hours of incubation, the culture medium was collected in a 50 mL centrifuge tube, and the cell pellet was collected in another tube with lysis buffer (50 mM Tris-HCl, 150 mM NaCl, 2 mM MgCl 2 ...

Transfer the fine chopped tissue pieces in 10 mL plain DMEM containing Collagenase P (0.45 mg/mL) in 50 mL tubes. Keep the tubes in 37 C incubator ... then filter through 70 m strainer and centrifuge ...

Institute of Organic Chemistry and Biochemistry of the Czech Academy of Sciences, Flemingovo n. 2, Prague 6 16610, Czech Republic Department of Physical and Macromolecular Chemistry, Faculty of ...

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Peer ReviewDownload a summary of the editorial decision process including editorial decision letters, reviewer comments and author responses to feedback. The identification of tumour-specific ...

This acidic effluent produced during the mining process is called ‘Acid Mine Drainage’ (AMD) [5,6]. AMD is a major pollutant resulting in enhanced acidity of water bodies wherever it exists [7]. Acid ...

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