Gene expression studies give a snapshot of the different types of RNAs being expressed at a given time point in cells or tissues in vivo. Different RNA types have different roles, for example in ...

In this Teach Me in 10, Dr. Irene Sarkar from Thermo Fisher Scientific, introduces a real-time PCR detection software ...

Real-time quantitative PCR (RT-qPCR or qPCR) is revolutionizing many aspects of molecular biology and becoming the gold standard for accurate, sensitive, and fast quantification of gene expression.

Quantitative PCR (qPCR) technologies have exploded in the last decade. Commonly used for both diagnostics and basic research, new and exciting advances are being made in such areas as biomarker ...

Scientists around the world employ quantitative real-time PCR (qPCR) to measure the amount of particular nucleic acid sequences within a sample. While this technique is rapid, sensitive, and ...

RNAConnect, developer of enzymatic tools for visualizing and manipulating RNA, today announced the release of the UltraMarathonRT ® Two-Step RT-qPCR Kit, a next-generation tool for RNA researchers ...

Comparison of expression data requires normalization. The optimum normalization method depends on sample type, with the most common being to normalize to reference genes. It is critical to select ...

Optimizing the formulation of reagents for your qPCR assay requires careful experimental design that looks across several performance factors for each reagent formulation tested. These performance ...

When working in a lab, launching a quantitative PCR (qPCR) experiment demands a comprehensive understanding of complicated protocols, principles, and methodologies. Analyzing the large amounts of ...

Gene expression analysis using qPCR quantification of RNA transcripts expressed by cells or viruses plays an important role in the discovery and development of novel biotherapeutics. While widely used ...