Spatial transcriptomics is a technique that provides information about gene expression patterns within intact tissues. This technology employs various methodologies, including in situ sequencing (ISS) ...

Biological systems are inherently three-dimensional—tissues form intricate layers, networks, and architectures where cells interact in ways that extend far beyond a flat plane. To capture the true ...

A new spatial transcriptomic technology captures RNA patterns without requiring expensive imaging ...

Spatial transcriptomics provides a unique perspective on the genes that cells express and where those cells are located. However, the rapid growth of the technology has come at the cost of ...

Challenges and Prospects. Challenges and prospects faced by spatial transcriptomics itself and its application to the musculoskeletal system. AI Artificial intelligence, FFPE Formalin fixed and ...

Single-cell RNA transcriptomics allows researchers to broadly profile the gene expression of individual cells in a particular tissue. This technique has allowed researchers to identify new subsets of ...

Perturb-FISH aims to address both challenges. Because this technique harnesses imaging spatial transcriptomics (iST), it offers high resolution and scalability, as well as relatively low costs. To ...

Conventional transcriptomic techniques have revealed much about gene expression at the population and single-cell level—but they overlook one crucial factor: spatial context. In musculoskeletal ...

Initially, cells are dissociated from liver tissues into single-cell suspensions using in vivo enzymatic perfusion or ex vivo digestion methods. The cells of interest are then enriched from the ...