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WEBExplore BioTechniques. A world leader in life science method research news. ISSN (print): 0736-6205 | ISSN (online): 1940-9818.
Genetically Engineered Cells used for Targeted Delivery
WEBJan 31, 2022 · Enucleated genetically engineered ‘cargo’ cells could provide the solution to challenging safety concerns raised by using heavily engineered stem cells for drug delivery. By removing the nucleus from mesenchymal stromal stem cells, a combined team from the School of Medicine and Moores Cancer Centre at the University of California, San ...
Memory Manipulation: The Key to Treating PTSD? - BioTechniques
WEBMay 30, 2019 · Manipulating memories. By activating and suppressing regions of a mouse hippocampus, researchers have found a way to manipulate memories, potentially giving rise to new PTSD treatment. The hippocampus has long been known to play an important role in the formation and storage of memories. Each memory we have is …
Western Blotting Summary - BioTechniques
WEBWestern blotting (or immunoblotting) is a well-established biochemical technique that detects specific proteins in complex samples and has applications from basic research to the diagnosis of infectious diseases. Western blotting can be used to determine quantity, molecular weight and post-translational modifications of proteins and can ...
Emulsion PCR made easy - BioTechniques
WEBHere we describe a simplified method for direct breakage of the emulsion using the DNA binding buffer (containing guanidine hydrochloride and isopropanol) that is provided with commonly used silica column-based purification kits, followed by purification of the PCR products using silica columns. This method eliminates the routinely performed multi-step breakage of emulsion and extraction of […]
Nanopore sequencing: how does it work? - BioTechniques
WEBOxford Nanopore sequencing devices use flow cells that contain tiny holes – nanopores – embedded in an electro-resistant membrane. When DNA passes through a nanopore, the current is disrupted, producing a ‘squiggle’ that is specific to the particular base that has passed through in that moment. These squiggles can be decoded using ...
What is Western Blotting? The Story of the Western Blot
WEBJul 18, 2018 · The technique involves the identification and characterization of DNA-binding proteins using DNA probes (Southern) and protein blotting (western) and was first described in 1980. This method was published before the western blot. Yet, it still alludes to it in its name, reaffirming the popularity of the western blot.
BioTek Introduces New Cell Count & Viability Starter Kit
WEBApr 6, 2020 · The new Cell Count & Viability Starter Kit automates cell counting and viability measurements in a mammalian cell suspension. When used with BioTek’s Cytation™ Cell Imaging Multi-Mode Readers or Lionheart™ Automated Imagers in a workflow, researchers may quickly and automatically image and analyze cell counts in multiple samples. The ...
Sylvia Boj on Organoids for Drug Discovery - BioTechniques
WEBJun 8, 2020 · Sylvia Boj on gene editing in organoids and their use in drug discovery. In this interview we catch up with Sylvia Boj, who takes us through a recent paper that used patient-derived organoids as a test case for a new CRISPR technique and talks about the impact this technology could have in the development of …
Coffee Chat: Advanced proteomic analysis - BioTechniques
WEBThe Moritz group develops and applies cutting-edge proteomics technologies to biomarker analysis of wellness and aging, disease biomarkers for early detection of cancer and a number of infectious disease pathogens. The Moritz group is the primary developer of the world-renowned Trans-Proteomic Pipeline proteomics software tools for statistical ...